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ccl13  (R&D Systems)


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    R&D Systems ccl13
    Ccl13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ccl13+488/Human+CCL13%2FMCP-4+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pmc12903089-247-40-41
    Average 94 stars, based on 4 article reviews
    ccl13 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Staining:

    Article Title: A CRISPRi/a platform in iPSC-derived microglia uncovers regulators of disease states
    Article Snippet: Cells were fixed and permeabilized with the eBioscience Intracellular Fixation and Permeabilization Buffer Set (Invitrogen; Cat. No. 88-8824-00) according to the manufacturer’s instructions. .. Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems; Cat. No. IC002G) and Mouse IGG1 kappa Isotype (eBioscience; Cat. No. 50-4714-82) over night at 4 °C. .. Cells were washed twice with DPBS before analyzing them by flow cytometry using the BD FACS CelestaTM (BD Biosciences) or the BD FACSAria Fusion.

    Article Title: A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states
    Article Snippet: Cells were fixed and permeabilized with the eBioscience Intracellular Fixation and Permeabilization Buffer Set (Invitrogen; Cat. No. 88-8824-00) according to the manufacturer’s instructions. .. Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems; Cat. No. IC002G) and Mouse IGG1 kappa Isotype (eBioscience; Cat. No. 50-4714-82) overnight at 4 °C. .. Cells were washed twice with DPBS before analyzing them by flow cytometry using the BD FACS Celesta (BD Biosciences) or the BD FACSAria Fusion using BD FACSDiva (v.8.0.1.1) software.

    Article Title: A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states.
    Article Snippet: For intracellular protein staining, dissociated iTF-Microglia were fixed and permeabilized with the eBioscience Intracellular Fixation and Permeabilization Buffer Set (Invitrogen; Cat. No. 88-8824-00) according to the manufacturer's instructions. .. Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems; Cat. No. IC002G) and Mouse IGG1 kappa Isotype (eBioscience; Cat. No. 50-4714-82) over night at 4°C. ..

    Article Title: A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states.
    Article Snippet: Cells were fixed and permeabilized with the eBioscience Intracellular Fixation and Permeabilization Buffer Set (Invitrogen; Cat. No. 88- 8824-00) according to the manufacturer’s instructions. .. Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems; Cat. No. IC002G) and Mouse IGG1 kappa Isotype (eBioscience; Cat. No. 50-4714-82) overnight at 4 °C. .. Cells were washed twice with DPBS before analyzing them by flow cytometry using the BD FACS Celesta (BD Biosciences) or the BD FACSAria Fusion using BD FACSDiva (v.8.0.1.1) software.

    Control:

    Article Title: A CRISPRi/a platform in iPSC-derived microglia uncovers regulators of disease states
    Article Snippet: Cells were fixed and permeabilized with the eBioscience Intracellular Fixation and Permeabilization Buffer Set (Invitrogen; Cat. No. 88-8824-00) according to the manufacturer’s instructions. .. Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems; Cat. No. IC002G) and Mouse IGG1 kappa Isotype (eBioscience; Cat. No. 50-4714-82) over night at 4 °C. .. Cells were washed twice with DPBS before analyzing them by flow cytometry using the BD FACS CelestaTM (BD Biosciences) or the BD FACSAria Fusion.

    Article Title: A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states
    Article Snippet: Cells were fixed and permeabilized with the eBioscience Intracellular Fixation and Permeabilization Buffer Set (Invitrogen; Cat. No. 88-8824-00) according to the manufacturer’s instructions. .. Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems; Cat. No. IC002G) and Mouse IGG1 kappa Isotype (eBioscience; Cat. No. 50-4714-82) overnight at 4 °C. .. Cells were washed twice with DPBS before analyzing them by flow cytometry using the BD FACS Celesta (BD Biosciences) or the BD FACSAria Fusion using BD FACSDiva (v.8.0.1.1) software.

    Article Title: A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states.
    Article Snippet: For intracellular protein staining, dissociated iTF-Microglia were fixed and permeabilized with the eBioscience Intracellular Fixation and Permeabilization Buffer Set (Invitrogen; Cat. No. 88-8824-00) according to the manufacturer's instructions. .. Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems; Cat. No. IC002G) and Mouse IGG1 kappa Isotype (eBioscience; Cat. No. 50-4714-82) over night at 4°C. ..

    Article Title: A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states.
    Article Snippet: Cells were fixed and permeabilized with the eBioscience Intracellular Fixation and Permeabilization Buffer Set (Invitrogen; Cat. No. 88- 8824-00) according to the manufacturer’s instructions. .. Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems; Cat. No. IC002G) and Mouse IGG1 kappa Isotype (eBioscience; Cat. No. 50-4714-82) overnight at 4 °C. .. Cells were washed twice with DPBS before analyzing them by flow cytometry using the BD FACS Celesta (BD Biosciences) or the BD FACSAria Fusion using BD FACSDiva (v.8.0.1.1) software.



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